Lub hom phiaj tseem ceeb ntawm cov ntshav ntshav khaws cov hlab ntshav yog txhawm rau muab cov qauv ntshav zoo thiab zoo tshaj plaws. Hom raj nqus ntshav nqus cov raj no feem ntau siv nrog cov raj ntsuab, cov raj xiav thiab cov raj txho. Lub taub hau ntsuab siv heparin sodium lossis ntsev lub cev ua anticoagulant, uas feem ntau siv rau kev kuaj ntshav sai, kuaj ntshav rheology, thiab lwm yam. Lub taub hau lub raj raj muaj 0.109mol/L sodium citrate buffer system ua anticoagulant. Anticoagulant thiab Qhov ntim ntawm cov ntshav yog 1: 9. Nws yog siv los ntsuas ntshav coagulation. Lub taub hau grey muaj cov ntshav qabzib nyob ruaj khov sodium nitrate thiab anticoagulant potassium oxalate lossis EDTA. Nws yog siv los ntsuas ntshav qab zib.
1. Qhov siab ntawm heparin hauv lub raj taub hau ntsuab yuav tsum yog 12-30USP/ml. Yog tias nws qis dua qhov txwv qis, cov ntshav me me yuav tshwm. Thaum nws siab dua qhov txwv saum toj no, kev rog yuav ua rau cov qe ntshav liab hauv cov ntshav nqaim, thiab cov tshuaj hauv cov qe ntshav liab yuav raug tso rau hauv cov ntshav thiab ua rau muaj qhov txawv txav ntawm ntau yam tshuaj lom neeg.
2. Qhov ntim sib piv ntawm sodium citrate tov rau cov ntshav hauv lub raj taub hau xiav yuav tsum yog 1: 9. Vim tias cov ntsiab lus ntawm sodium citrate anticoagulant yuav cuam tshuam rau cov ntsiab lus calcium ions hauv cov ntshav, thiab cov txiaj ntsig ntawm Peng Xiang cov ntshav coagulation kuaj , thaum Thaum sib piv cov ntshav rau 0.109M sodium citrate tov tau txo los ntawm 9: 1 txog 7: 1, APTT qhov txiaj ntsig ntsuas yuav nce ntxiv: thaum nws poob mus rau 4.5: 1, qhov ntsuas PT yuav muaj qhov hloov pauv tseem ceeb.
3. Lub silicification ntawm lub raj phab ntsa ntawm lub raj taub hau xiav yog ib qho tseem ceeb heev. Nws nyob ntawm seb cov chaw tsim khoom' txheej txheem kev tsim khoom tau nce qib. Hauv kev kho mob, kev ntsuas ntshav coagulation xav tau txo qis hauv kev nqus coagulation yam ntawm phab ntsa raj. Ib qho ntxiv, thaum xaiv cov yas yas pob txha taub hau, them nyiaj tshwj xeeb rau seb cov chaw tsim khoom puas muaj cov raj tshuab ua cov tshuab. Tsis ntev los no, ib lub tshuab nqus tsev siv hauv tsev kho mob Ntshav ntshav hlab ntshav (lub raj yas yas uas muaj 0.109M sodium citrate) tau pom tias siv tsis tau vim yog qhov tseeb tias cov tshuaj anticoagulant hauv lub raj tau txo qis, thiab qee lub raj pom kua anticoagulant. Qhov laj thawj yog tias cov ntshav khaws cov pob txha raj phab ntsa ua tiav. Vim tias cov khoom siv ntawm lub raj yog ua los ntawm cov khoom PET. Nws yog hydrophilic. Yog tias tuaj yeem tsim daim nyias nyias hydrophobic thaum lub raj phab ntsa ua tiav, qhov xwm txheej saum toj no yuav tshwm sim. Qhov kev xeem tsis tuaj yeem lav tau. Qhov tseeb ntawm qhov tshwm sim.
4. Cov tsoos tsho taub hau grey feem ntau siv cov tshuaj potassium oxalate-sodium ua cov tshuaj tiv thaiv kab mob. Raws li cov tshuaj potassium oxalate anticoagulant tau yooj yim ua rau hemolysis ib txwm, nws raug nquahu kom siv cov sodium fluoride-disodium ethylenediaminetetraacetic acid sib xyaw ua ke. Nws tuaj yeem tswj hwm kev ruaj ntseg ntawm cov ntshav thiab zam kev pheej hmoo ntawm exogenous coagulation thiab hemolysis.
