1.1 Cov kauj ruam ua haujlwm
Ntxiv 5 mL ntawm cov qauv piv rau 50 mL, kua txiv hmab txiv ntoo kua txiv hmab txiv ntoo, thiab muab tso rau ntawm 35 ° C ± 2' C rau 24 h.
Cov kab lis kev cai tau nthuav tawm thiab tsis muaj kab mob rau ntawm cov phaj agar ntshav, ua kab lis kev cai ntawm 35 ℃ ± 2' C rau 24 teev kom pom cov yam ntxwv ntawm pawg neeg. Cov hemolytic streptococcus tawm dawb-dawb ntawm cov phaj ntshav, txhais tau tias tsis pom lossis tsis pom, nrog rab koob zoo li qhov ua paug, nto du, zoo huv si, thiab tsis muaj xim pob tshab hemolysis puag ncig nws.
Xaiv cov cheeb tsam ib puag ncig rau smear Gram staining microscopy. Lawv yuav tsum yog Gram-positive thiab zoo li cov saw hlau. Yog tias kev kuaj lub tshuab me me ua tau raws li cov xwm txheej saum toj no, cov kev ntsuas hauv qab no yuav tsum tau ua:
Kev ntsuam xyuas Streptokinase: Kos 0.2 mL ntawm cov tshuaj potassium oxalate ntshav (0.01 g potassium oxalate thiab 5 mL luav ntshav tau sib xyaw, centrifuged kom nag, thiab supernatant tau kos). Ntxiv 0.8 mL cov kua qaub uas tsis muaj menyuam, sib xyaw kom zoo, thiab tom qab ntawd ntxiv rau qhov kev xeem 24 h cov kab lis kev cai kab mob 0.5 ml, thiab 0.25% calcium chloride 0.25 mL, sib tov zoo, muab tso rau hauv 3510 ± 2 bath dej da dej, saib ib zaug 2 feeb (feem ntau, nws tuaj yeem coagulated tsis pub dhau 10 feeb), thiab tos kom cov ntshav coagulate Tom qab ntawv txuas ntxiv mus saib thiab sau lub sijhawm yaj. Yog tias nws tsis yaj hauv 2 teev, txuas ntxiv tso rau 24 teev kom pom. Yog tias cov hlab ntsha tawg tag, nws zoo, thiab yog tias nws tsis yaj hauv 24 teev, nws tsis zoo.
Bacillus daim tawv nqaij rhiab rhiab: kis cov kab mob sib kis ua kua rau hauv cov ntshav, siv tus menyuam ntxais kom noj txhua daim ntawv uas muaj 0.04 units ntawm bacillus peptide thiab muab tso rau saum lub phaj. Nyob rau tib lub sijhawm, siv qhov kev paub zoo uas zoo li kev tswj hwm ntawm 35 ℃. Muab tso rau ntawm ± 2 ° C rau 18-24 h, cov kab mob bacteriostatic zoo.
1.2 Cov ntawv tshaj tawm
Kev tshuaj xyuas me me ntawm cov gram-positive chain array ball M, cov ntshav qhia pom lub voj voos hemolytic, streptokinase thiab tus pas ntsuas M1 limb yog qhov zoo, tuaj yeem tshaj qhia txog kev kuaj pom hemolytic streptococcus hauv tus qauv.
