1. Lub teeb ntsuab lub taub hau npog ntawm cov ntshav sib cais raj, ntxiv heparin anticoagulant rau hauv lub raj sib cais inert, tuaj yeem ua tiav lub hom phiaj ntawm kev sib cais ntshav sai. Nws yog siv rau kev ntsuas thiab ntsuas ntshav ntshav ntshav thaum muaj xwm ceev xws li ICU. Cov ntshav ntshav tuaj yeem tso ncaj qha rau ntawm lub tshuab thiab khaws ruaj khov rau 48 teev hauv qab tub yees.
2. EDTA anticoagulation tube ntshav cap, ethylenediaminetetraacetic acid ((EDTA, hnyav hnyav 292) thiab nws cov ntsev yog ib hom aminopolyacid, uas tuaj yeem ua tau zoo chelate calcium ions hauv cov qauv ntshav, chelate calcium lossis tshem tawm cov tshuaj tiv thaiv calcium hauv qhov chaw yuav thaiv thiab ua kom tiav cov txheej txheem endogenous lossis exogenous coagulation, yog li tiv thaiv cov ntshav los ntawm kev txhaws. creatine kinase thiab leucine aminopeptidase txiav txim siab thiab kuaj PCR.
3. Lub raj tshuaj sodium cumelate coagulation muaj lub hau xiav lub teeb. Sodium glutathione feem ntau ua raws li anticoagulant los ntawm chelating nrog calcium ions hauv cov ntshav. Nws yog qhov tsim nyog rau kev sim ntshav coagulation. Cov tshuaj anticoagulant tau pom zoo los ntawm Pawg Neeg Soj Ntsuam rau cov qauv kuaj sim (NCCLS) yog 3.2% lossis 3.8% (sib npaug li 0.109mol/domain 0.129mol/L). Cov ntshav sib piv yog 1: 9.
4. Sodium citrate erythrocyte sedimentation rate test tube nrog lub hau dub, sodium citrate concentration xav tau rau erythrocyte sedimentation tus nqi ntsuas yog 3.2% (sib npaug rau 0.109mol/L) Qhov piv ntawm anticoagulant rau ntshav yog 1: 4
5. Potassium oxalate/sodium fluoride grey lub hau npog. Sodium fluoride yog cov tshuaj tiv thaiv tsis muaj zog. Nws feem ntau yog siv ua ke nrog potassium oxalate lossis sodium diiodate. Qhov sib piv yog 1 feem ntawm sodium oxalate thiab 3 feem ntawm potassium oxalate. . 4mg ntawm qhov sib xyaw no tuaj yeem tiv thaiv 1ml ntshav los ntawm coagulating thiab inhibiting decomposition hauv 23 hnub. Nws yog ib qho kev tiv thaiv zoo rau kev txiav txim siab cov piam thaj hauv ntshav. Nws tsis tuaj yeem siv rau kev txiav txim siab ntawm urea los ntawm txoj kev urease, lossis nws yog siv rau kev txiav txim siab ntawm alkaline phosphatase thiab amylase. , Pom zoo rau kev ntsuas ntshav qabzib.
Siv heparin anticoagulation lub raj nqus dej thiab lub raj nqus tsis-anticoagulation los sau txog 7 ml ntshav los ntawm anterior vena cava, centrifuge ntawm 3500 rpm rau 10 min cais cov ntshav thiab ntshav, thiab khaws cia ntawm 80 degrees rau kev tshuaj xyuas;
1 mL ntawm cov ntshav tau sau los ntawm vena cava nrog EDTA K3 anticoagulation tube rau kev faib cov qe ntshav dawb.
7 ml ntawm cov ntshav tau sau los ntawm lub hauv ntej vena cava nrog heparin anticoagulation lub tshuab nqus tsev raj. Qhov kev hloov pauv ntawm cov ntshav sab hauv lymphocytes tau txiav txim siab.
